Increased Expression of Tenascin-X in Thoracic and Abdominal Aortic Aneurysm Tissues

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Author(s)

    • Satoh Kazumi Satoh Kazumi
    • Department of Biosignaling and Radioisotope Experiment, Center for Integrated Research in Science, Shimane University
    • Totsuka Yasunori
    • Department of Oral and Maxillofacial Surgery, Graduate School of Dental Medicine, Hokkaido University
    • Oda Teiji
    • Division of Cardiovascular and Thoracic Surgery, Department of Surgery, Faculty of Medicine, Shimane University
    • Matsumoto Ken-ichi
    • Department of Biosignaling and Radioisotope Experiment, Center for Integrated Research in Science, Shimane University

Abstract

Tenascin-X (TNX), which has a molecular mass of roughly 450 kDa, is the largest member of the tenascin family. Complete deficiency of TNX in humans leads to a recessive form of Ehlers–Danlos syndrome (EDS). TNX is expressed abundantly in a variety of tissues, especially in cardiac muscle and in perivascular stroma. Human TNX is also present in serum with an apparent molecular size of 140 kDa. In the present study, we investigated the expression levels of TNX protein in thoracic and abdominal aortic aneurysm tissues. The level of TNX was significantly increased in both aortic aneurysm tissues compared with that in adjacent normal tissues. Next, to compare TNX levels in serum from both patients with thoracic aortic aneurysm and patients with abdominal aortic aneurysm with levels in serum from healthy individuals, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) using TNX-specific antibodies. Measurement of TNX serum concentrations in both aortic aneurysm patients and controls showed that the levels were almost the same. These results indicate that TNX expression is significantly elevated in both thoracic and abdominal aortic aneurysm tissues but that the increase in TNX levels in both tissues does not result in an increase in TNX serum concentration in patients with TAA or AAA.

Journal

  • Biological and Pharmaceutical Bulletin

    Biological and Pharmaceutical Bulletin 33(11), 1898-1902, 2010

    The Pharmaceutical Society of Japan

Codes

  • NII Article ID (NAID)
    130000402314
  • NII NACSIS-CAT ID (NCID)
    AA10885497
  • Text Lang
    ENG
  • ISSN
    0918-6158
  • NDL Article ID
    10859152
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-V41
  • Data Source
    NDL  J-STAGE 
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