The Influence of Dietary Restriction on the Development of Diabetes and Pancreatitis in Female WBN/Kob-Fatty Rats

• AKIMOTO Toshio

  Division of Laboratory Animal Science, Nippon Medical School

• TERADA Misao

  Division of Laboratory Animal Science, Nippon Medical School

• SHIMIZU Akira

  Department of Analytic Human Pathology, Nippon Medical School

• SAWAI Nobuhiko

  Department of Anatomy and Neurobiology, Nippon Medical School

• OZAWA Hitoshi

  Department of Anatomy and Neurobiology, Nippon Medical School

Abstract

Original WBN/Kob male rats commonly develop chronic pancreatitis by the age of 3 months, while diabetes mellitus occurs at 9 months. In contrast, female rats of this strain do not show pancreatitis or diabetes. The WBN/Kob-fatty rat is a homozygous (fa/fa) congenic strain for the fa allele of the leptin receptor gene (Lepr). In WBN/Kob-fatty rats, both females and males provide a model of non-insulin-dependent diabetes with obesity. The leptin receptor fatty gene (Lepr^fa) induces obesity and hyperphagia. In the present study, we examined the effect of dietary restriction on pancreatitis and diabetes in female WBN/Kob-fatty rats. Five female fatty rats comprised a restricted feeding group with paired-feeding from 3 to 13 weeks of age, and five female lean rats comprised a control group with paired-feeding. At 13 weeks of age, two of the five female fatty rats of the control group developed diabetes mellitus, while no female fatty rats of the restricted feeding group developed diabetes mellitus. At this stage, pathological changes of the pancreas were observed in female fatty rats. All female fatty rats showed severe interlobular, intra-lobular and intra-islet fibrosis. In female fatty rats of the restricted feeding group, pathological changes of the pancreas were milder those of the free-feeding fatty group. Although dietary restriction could not completely prevent pancreatitis in female fatty rats, the development of diabetes was inhibited by its reduction of the severity of pancreatitis.<br>

Journal

• Experimental Animals

  Experimental Animals 59 (5), 623-630, 2010

  Japanese Association for Laboratory Animal Science

Keywords

• diabetes
• leptin receptor
• obesity
• pancreatitis
• WBN/Kob rat

Details

• CRID: 1390001205044469632
• NII Article ID: 130000419257
• NII Book ID: AA11032321
• DOI: 10.1538/expanim.59.623
• ISSN: 18817122
• ISSN: 00075124
• ISSN: 13411357
• NDL BIB ID: 10838233
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10838233
• Web Site: http://www.jstage.jst.go.jp/article/expanim/59/5/59_5_623/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011119083
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed