False Positive Responses of Campylobacter jejuni when Using the Chemical-In-Plug Chemotaxis Assay

• KANUNGPEAN Doungjit

  Laboratory of Animal Hygiene, School of Veterinary Medicine, Kitasato University

• KAKUDA Tsutomu

  Laboratory of Animal Hygiene, School of Veterinary Medicine, Kitasato University

• TAKAI Shinji

  Laboratory of Animal Hygiene, School of Veterinary Medicine, Kitasato University

Abstract

Campylobacter jejuni reportedly exhibits chemotactic behavior towards fucose, several amino acids and organic acids, mucin and bile. The chemotaxis of C. jejuni has mainly been studied using the chemical-in-plug chemotaxis assay. In this study, a nonchemotactic mutant (cheY mutant) and nonmotile mutant (flhA mutant) were constructed and used as negative controls in an assay. Apparent zones of accumulation around test plugs containing several amino acids and organic acids were observed with both of the mutants. Our results suggest that positive responses of C. jejuni in the chemical-in-plug assay are not always indicative of chemotaxis.<br>

Journal

• Journal of Veterinary Medical Science

  Journal of Veterinary Medical Science 73 (3), 389-391, 2011

  JAPANESE SOCIETY OF VETERINARY SCIENCE

Keywords

• <i>Campylobacter jejuni</i>
• chemical-in-plug assay
• chemotaxis

Details

• CRID: 1390001206428308096
• NII Article ID: 130000444303
• DOI: 10.1292/jvms.10-0396
• ISSN: 13477439
• ISSN: 09167250
• Web Site: http://www.jstage.jst.go.jp/article/jvms/73/3/73_10-0396/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2011186331
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed