Genomic Diversity among Equine Herpesvirus-4 Field Isolates
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- MAEDA Ken
- Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University
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- KAI Kazushige
- Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University
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- MATSUMURA Tomio
- Epizootic Research Station, Equine Research Institute, Japan Racing Association
Bibliographic Information
- Other Title
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- ウマヘルペスウイルス4型野外株間の遺伝子の多様性
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Abstract
Infection with equine herpesvirus-4 (EHV-4) is a major cause of respiratory tract disease, equine rhinopneumonitis, in horses. Although the full sequence of EHV-4 has been reported, genomic differences among EHV-4 field isolates have not yet been characterized. In this study, the genomic diversity between 23 Japanese EHV-4 isolates was analyzed by digestion with restriction endonucleases (BamHI, BgIII, EcoRI, SacI, and SalI) and polymerase chain reaction (PCR). The restriction endonuclease digestion patterns of the EHV-4 field isolates showed distinct differences which included mobility shifts of some fragments as well as loss and/or gain of fragments. Two EHV-4 genes containing repeat sequences, ORFs 24 and 71, were amplified by PCR and the amplified fragments were compared among the field isolates. The sizes of the amplified fragments varied among epizootiologically unrelated isolates, while the fragments of related isolates had the same size. The observed genomic diversity among EHV-4 field isolates may be a useful tool for epidemiological study of equine rhinopneumonitis by EHV-4 infection.<br>
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 67 (6), 555-561, 2005
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390282681401572992
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- NII Article ID
- 130000448257
- 110003963769
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- NII Book ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 7403864
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- PubMed
- 15997181
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed