Method for Estimation of Toxic Endotoxin in Inactivated Salmonella Vaccine in D-Galactosamine-Sensitized Mice

• TAKIKAWA Noriyasu

  Research Center for Biologicals, The Kitasato Institute

• KAWAHARA Kazuyoshi

  Research Center for Biologicals, The Kitasato Institute

• SAWATA Akira

  Research Center for Biologicals, The Kitasato Institute

• KUME Katsumi

  Research Center for Biologicals, The Kitasato Institute

Abstract

We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the injection. The best result was obtained when C3H/HeN mice were used for the test. Correlation was observed between the endotoxin content measured by Limulus amoebocyte lysate assay and the LD₅₀ in the mouse safety test (r=0.81). These results suggested that this test could be applied to the estimation of endotoxin content in inactivated vaccines of Salmonella.<br>

Journal

• Journal of Veterinary Medical Science

  Journal of Veterinary Medical Science 66 (12), 1591-1593, 2004

  JAPANESE SOCIETY OF VETERINARY SCIENCE

Keywords

• endotoxin
• <i>Salmonella</i>
• vaccine

Details

• CRID: 1390001206424704896
• NII Article ID: 110003963656
• NII Article ID: 130000448694
• NII Article ID: 10014192660
• NII Book ID: AA10796138
• DOI: 10.1292/jvms.66.1591
• ISSN: 13477439
• ISSN: 09167250
• NDL BIB ID: 7196434
• PubMed: 15644614
• Web Site: http://id.ndl.go.jp/bib/7196434
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7196434
• Web Site: http://www.jstage.jst.go.jp/article/jvms/66/12/66_12_1591/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005081874
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed