Prevalence of Porcine Endogenous Retrovirus in Domestic Pigs in Japan and Its Potential Infection in Dogs Xenotransplanted with Porcine Pancreatic Islet Cells
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- EDAMURA Kazuya
- Laboratories of Veterinary Emergency Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University
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- NASU Koko
- Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University
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- IWAMI Yukiko
- Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University
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- NISHIMURA Ryohei
- Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- OGAWA Hiroyuki
- Laboratories of Veterinary Emergency Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- SASAKI Nobuo
- Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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- OHGAWARA Hisako
- Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University
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抄録
The prevalence of porcine endogenous retrovirus (PERV) proviral DNA among various pig breeds raised in Japan was investigated by polymerase chain reaction (PCR). Moreover, potential infection of PERV was investigated by PCR and reverse transcriptase-polymerase chain reaction (RT-PCR) in experimentally induced diabetic dogs (n=5) implanted with the diffusion chamber type bio-artificial endocrine pancreas (Bio-AEP) containing porcine pancreatic endocrine (PE) cells. No immunosupplessant was used after the transplantation. PERV gag, pol, env-A and env-B genes were detected in any pigs examined. In two of three Landrace breeds, env-C gene was absent. PERV proviral DNAs and viral RNAs were also detected from the cultured porcine PE-cells. In the peripheral blood mononuclear cells and the spleen obtained at 6, 30, 32, 36, 79 weeks of xenotransplantation in dogs, however, no evidence of microchimerism, infection and viremia were confirmed. These results suggested that the risk of PERV infection through xenotransplantation of Bio-AEP containing porcine islet cells without immunosuppressants may be quite low.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 66 (2), 129-135, 2004
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206426675712
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- NII論文ID
- 110003886368
- 130000448705
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 6864029
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- PubMed
- 15031539
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可