TRAIL-Decoy Receptor 1 Plays Inhibitory Role in Apoptosis of Granulosa Cells from Pig Ovarian Follicles.
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- WADA Satoko
- Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
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- MANABE Noboru
- Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
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- NAKAYAMA Mizuho
- Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
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- INOUE Naoko
- Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
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- MATSUI Toshikatsu
- Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
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- MIYAMOTO Hajime
- Unit of Anatomy and Cell Biology, Department of Animal Sciences, Kyoto University
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抄録
Previously, we histochemically examined the localization of tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and its receptors in porcine ovarian follicles, and demonstrated a marked reduction in the expression of TRAIL-decoy receptor-1 (DcR1) in granulosa cells of atretic follicles. In the present study, to confirm the inhibitory activity of DcR1 in granulosa cells, granulosa cells prepared from healthy follicles were treated with phosphatidylinositol-specific phospholipase C (PI-PLC) to cleave glycophospholipid anchor of DcR1 and to remove DcR1 from the cell surface, and then incubated with TRAIL. PI-PLC treatment increased the number of apoptotic cells induced by TRAIL. The present finding indicated the possibility that TRAIL and its receptors were involved in induction of apoptosis in granulosa cells during atresia, and that DcR1 plays an inhibitory role in granulosa cell apoptosis.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 64 (5), 435-439, 2002
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206425539328
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- NII論文ID
- 130000449280
- 110003920871
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD38Xlt1arsbo%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 6151705
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- PubMed
- 12069077
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可