Application of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Discrimination of Escherichia Strains Possessing Highly Conserved Ribosomal RNA Gene Sequences

• Muroi Masashi

  Research Institute of Pharmaceutical Sciences, Musashino University

• Shima Keisuke

  Analytical & Measuring Instruments Division, Shimadzu Corporation

• Nakagawa Yasuyoshi

  Resource Collection Division, National Institute of Technology and Evaluation

• Tanamoto Ken-ichi

  Research Institute of Pharmaceutical Sciences, Musashino University

抄録

We evaluated the capability of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) to discriminate twelve Escherichia strains: E. blattae, E. fergusonii, E. hermanii and nine E. coli, whose ribosomal RNA (rRNA) gene sequence homologies are in the range of 96—100%. Similarities obtained by MALDI-TOF MS were found to be 78—92% among the E. coli strains, and 74% between E. coli and E. fergusonii. E. blattae and E. hermanii showed only 32% similarity when compared to the other species. Thus, MALDI-TOF MS provides capability of distinguishing bacterial species or even strains possessing highly conserved rRNA gene sequences.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 34 (3), 430-432, 2011

  公益社団法人 日本薬学会

キーワード

• mass spectrometry
• ribosomal RNA
• <i>Escherichia</i>

詳細情報

• CRID: 1390001204627499136
• NII論文ID: 130000657825
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.34.430
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 10990185
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I10990185
• Web Site: http://www.jstage.jst.go.jp/article/bpb/34/3/34_3_430/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012000205
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可