腐肉食性巻貝キンシバイNassarius (Alectrion) glansに認められたフグ毒の毒性と毒成分  [in Japanese] Toxicity and Toxin Profile of Tetrodotoxin Detected in the Scavenging Gastropod Nassarius (Alectrion) glans "Kinshibai"  [in Japanese]

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Abstract

2007年9月~2008年1月に長崎県橘湾で採集した小型巻貝7種計66個体につき,マウス試験で毒性を調べたところ,キンシバイは供試した22個体すべてが有毒であった.毒力は極めて強く,特に2007年9月に採集したものでは,10個体中8個体の筋肉または内臓が1,000 MU/gを上回った.興味深いことに,22個体中13個体で筋肉の総毒力(725~9,860 MU/個体)が内臓よりも5.9~110倍高い値を示した.LC/MS分析により,毒の主成分は tetrodotoxin (TTX) であることが明らかとなり,加えて11-oxoTTXを含むことが示唆された.一方,同時期同海域で採取したその他の巻貝については,毒性は全く認められなかった(5 MU/g未満).

From September 2007 to January 2008, a total of 66 specimens of 7 gastropod species, <i>Nassarius</i> (<i>Alectrion</i>)<i> glans</i> (<i>n</i>=22), <i>Bufonaria rana</i> (<i>n</i>=11), <i>Ficus subintermedia</i> (<i>n</i>=10), <i>Stellaria</i> (<i>Onustus</i>) <i>exutus</i> (<i>n</i>=8), <i>Tonna luteostoma</i> (<i>n</i>=7), <i>Hemifusus tuba</i> (<i>n</i>=4) and <i>Semicassis bisulcata persimilis</i> (<i>n</i>=4), were collected from Tachibana Bay, Nagasaki Prefecture, Japan, and their toxicity was determined by mouse bioassay. Among the gastropods tested, all <i>N</i>. <i>glans </i> specimens were toxic, whereas no other species showed toxicity of more than 5 MU/g. The toxicity scores of <i>N. glans</i> were very high; 48-2,730 MU/g(775±615 MU/g)in the muscle, and 16-10,200 MU/g(1,490±2,530 MU/g)in the viscera, including digestive gland. Interestingly, toxin was localized in the muscle in 13 of 22 specimens, where the total toxicity of the muscle (725-9,860 MU/individual) was 5.9-110 times higher than that of the viscera. LC/MS analysis demonstrated that the toxin of <i>N</i>. <i>glans</i> consisted mainly of TTX, which accounting for about 60-65% of the total toxicity. As for the remaining toxicity, participation of 11-oxoTTX was suggested. No paralytic shellfish poison was detected in HPLC-FLD analysis.

Journal

  • Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)

    Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 50(1), 22-28, 2009

    Japanese Society for Food Hygiene and Safety

Cited by:  1

Codes

  • NII Article ID (NAID)
    130000671630
  • NII NACSIS-CAT ID (NCID)
    AN00117741
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    0015-6426
  • NDL Article ID
    10216777
  • NDL Source Classification
    ZS17(科学技術--医学--衛生学・公衆衛生)
  • NDL Call No.
    Z19-364
  • Data Source
    CJPref  NDL  J-STAGE  JASI 
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