LC-MS/MSを用いた畜水産食品中のイミドカルブの分析 Analysis of Imidocarb in Livestock and Seafood Products Using LC-MS/MS

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Abstract

高速液体クロマトグラフ-タンデム質量分析計(LC-MS/MS)を用いた10種の畜水産食品中の抗原虫剤イミドカルブの簡便な分析法を検討した.カラムに TSK VMpak-25 を,移動相に酢酸アンモニウム-アセトニトリルを用い,ESI,ポジティブモードで測定した.分析に供する試験溶液は,試料を塩基性条件下,アセトニトリルで抽出し,調製した.はちみつ以外の試料には脂質などの除去のため,ヘキサンとの分配抽出を適用し,はちみつについては試料に添加した水と抽出有機溶媒との分離を改善するため,食塩による塩析操作を加えた.本法による定量下限値は食品中の濃度で0.005μg/gであり,回収率も76~109% と良好であった.また,併行精度および室内精度においても良好な結果が得られた.

A simple method using high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was investigated for the detection of the antiprotozoal drug imidocarb in 10 livestock and seafood products. Liquid chromatographic separation employed a TSK VMpak-25 column with ammonium acetate-acetonitrile as a mobile phase. Mass spectral acquisition was performed in the ESI positive-ion mode. Imidocarb was extracted from all samples using liquid extraction with acetonitrile under basic conditions. For samples other than honey, fat-soluble impurities were removed by acetonitrile-hexane partitioning. The salting-out technique was used for extraction from honey in order to improve the separation of the organic solvent and water added to the honey sample. The limit of quantitation was 0.005 μg/g (expressed as concentration in samples). The recoveries from all samples were 76-109%, and the repeatability and reproducibility were also satisfactory.

Journal

  • Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)

    Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 52(1), 34-39, 2011

    Japanese Society for Food Hygiene and Safety

Codes

  • NII Article ID (NAID)
    130000671809
  • NII NACSIS-CAT ID (NCID)
    AN00117741
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0015-6426
  • NDL Article ID
    11033357
  • NDL Source Classification
    ZS17(科学技術--医学--衛生学・公衆衛生)
  • NDL Call No.
    Z19-364
  • Data Source
    NDL  IR  J-STAGE 
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