A Novel Immunohistochemical Staining Method Allows Ultrarapid Detection of Lymph Node Micrometastases While Conserving Antibody

• Toda Hiroshi

  Department of Thoracic Surgery, Akita University Graduate School of Medicine

• Minamiya Yoshihiro

  Department of Thoracic Surgery, Akita University Graduate School of Medicine

• Kagaya Masami

  Akita Prefectural Research and Development Center

• Nanjo Hiroshi

  Division of Clinical Pathology, Akita University Hospital

• Akagami Yoichi

  Akita Prefectural Research and Development Center

• Saito Hajime

  Department of Thoracic Surgery, Akita University Graduate School of Medicine

• Ito Manabu

  Department of Thoracic Surgery, Akita University Graduate School of Medicine

• Konno Hayato

  Department of Thoracic Surgery, Akita University Graduate School of Medicine

• Motoyama Satoru

  Department of Thoracic Surgery, Akita University Graduate School of Medicine

• Ogawa Junichi

  Department of Thoracic Surgery, Akita University Graduate School of Medicine

抄録

We developed a novel ultrarapid immunohistochemical staining method in which an AC electric field is used to facilitate detection of tumor cells. Frozen sections of non-small cell lung cancer in lymph nodes were fixed in acetone for 2 min, after which they were incubated for 2 min with an anti-pancytokeratin antibody cocktail and then with EnVision^TM complex under an alternating current (AC) electric field. The sections were then incubated with a chromogen (3,3'diaminobenzidine) for 3 min and counterstained with hematoxylin. This method enabled detection of tumor cells in frozen sections in less than 15 min. In addition, we were able to reduce the amount of antibody used by more than 90% when the sections were incubated under the AC electric field for a longer period. This method could be a useful tool for frozen section diagnosis and research. Furthermore, with this method the cost of immunohistochemical staining can be reduced.<br>

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 44 (3), 133-139, 2011

  日本組織細胞化学会

キーワード

• immunohistochemical staining
• lymph node metastases
• micrometastases

詳細情報

• CRID: 1390282679840156416
• NII論文ID: 130000722777
• DOI: 10.1267/ahc.11006
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc/44/3/44_11006/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012169560
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
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