Involvement of Bradykinin in Trypsin-Induced Urinary Bladder Contraction in Cyclophosphamide-Treated Rats

• Shimizu Naoko

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

• Nakahara Tsutomu

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

• Tsuda Yo

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

• Mori Asami

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

• Sakamoto Kenji

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

• Ishii Kunio

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

Abstract

Protease-activated receptor-2 (PAR-2) is activated by serine proteases, such as trypsin and mast cell tryptase. Previous studies have demonstrated that both trypsin and PAR-2 activating peptide contract isolated rat urinary bladder preparations, however, the mechanisms are not fully understood. In the present study, we examined the role of bradykinin in contractions induced by trypsin and the PAR-2 agonist 2-furoyl-LIGRL-NH₂ in urinary bladders isolated from control or cyclophosphamide (CYP)-induced cystitis rats. The contractile effects of trypsin were significantly greater in the preparations obtained from CYP-treated rats than in those from controls. The bradykinin B2 receptor antagonist Hoe 140 did not affect trypsin-induced contractions in control rat bladders, whereas it significantly reduced the contractile effects of trypsin on bladders from CYP-treated rats. On the other hand, Hoe 140 failed to affect contractions induced by the PAR-2 agonist 2-furoyl-LIGRL-NH₂. These results suggest that the actions of trypsin on urinary bladders in cystitis rats are partly exerted through stimulation of bradykinin B2 receptor in a PAR-2-independent manner. This mechanism seems to be involved in the enhancement of trypsin-induced bladder contractions observed after induction of cystitis with CYP in rats.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 34 (7), 1122-1125, 2011

  The Pharmaceutical Society of Japan

Keywords

• bradykinin
• cyclophosphamide
• protease-activated receptor-2
• trypsin
• urinary bladder

Details

• CRID: 1390001204632693888
• NII Article ID: 130000738107
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.34.1122
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 11132805
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11132805
• Web Site: http://www.jstage.jst.go.jp/article/bpb/34/7/34_7_1122/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012151824
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed