Microdialysis Measurement of In Vivo Gonadotropin-Releasing Hormone Secretion in the Goat.

DOI Web Site Web Site 被引用文献3件
  • YAMAGUCHI Mitsuo
    Laboratory of Veterinary Reproduction Tokyo University of Agriculture and Technology
  • MANABE Yasuhiro
    Laboratory of Veterinary Reproduction Tokyo University of Agriculture and Technology
  • TANAKA Tomomi
    Laboratory of Veterinary Reproduction Tokyo University of Agriculture and Technology
  • MORI Yuji
    Laboratory of Veterinary Ethology, The University of Tokyo
  • HOSHINO Kunio
    Laboratory of Veterinary Reproduction Tokyo University of Agriculture and Technology

書誌事項

タイトル別名
  • Microdialysis Measurement of In Vivo Go

この論文をさがす

抄録

The microdialysis technique was applied to examine the pattern of gonadotropin-releasing hormone (GnRH) secretion in the mediobasal hypothalamus of the goat using a probe with a polycarbonate membrane. An in vitro recovery test showed that changes in the GnRH concentration in the microdialysis perfusate were highly correlated (r=0.996) with those in the external medium, and the relative recovery rate was 2.8% at a flow rate of 5μl/min. Then an in vivo study was carried out in ovariectomized goats which had been implanted stereotaxically with a microdialysis probe in the median eminence of the hypothalamus. Artificial cerebrospinal fluid was perfused and every 10 min fractions were collected under conscious and unrestrained conditions. Patterns of perfusate GnRH were pulsatile and closely associated with LH pulses in the peripheral circulation suggesting that the microdialysis technique can be successfully adopted for examining the neurosecretory activity of GnRH neurons in the conscious goat.

収録刊行物

被引用文献 (3)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ