ニワトリはい十二指腸粘膜刷子縁の膜脂肪酸組成と膜酵素活性誘導に及ぼすハイドロコーチゾンならびにビタミンＤ３投与の影響

• 高瀬 幸子

  静岡県立大学食品栄養科学部栄養学科

• 合田 敏尚

  静岡県立大学食品栄養科学部栄養学科

書誌事項

タイトル別名

• Effects of hydrocortisone and vitamin D3 on fatty acid composition of duodenal brush border membrane in chick embryos.
• ニワトリ ハイジュウニシ チョウ ネンマク サッシ エン ノ マク シボウサン

抄録

A study was conducted to investigate the effects of hydrocortisone and vitamin D₃ on the fatty acid composition of the duodenal brush border membranes in developing chick embryos, and to observe whether such effects on membrane lipids are related to the developmental induction of membranebound enzyme activity. Hydrocortisone was administered to embryos on days 14 and 17 of incubation, and vitamin D₃ was administered on day 14 of incubation. The duodenal brush border membranes were isolated from the pooled duodenal mucosa of 10 or 15 embryos on day 20 of incubation. The administration of hydrocortisone resulted in an increase of duodenal mucosa weight and DNA content of the duodenal mucosa in 20-day-old embryos. The embryonic membrane protein in the duodenal brush border was also increased through this treatment. However, there was no increase of membrane protein as expressed per unit of mucosal DNA. Administration of vitamin D₃ had no effects on these parameters. The fatty acid composition of the duodenal brush border membranes was changed by both hydrocortisone and vitamin D₃ administration. The polyunsaturated fatty acid levels of ω6 acids such as 18: 2 (ω6) and 20: 4 (ω6) were significantly increased by both treatments. Hydrocortisone administration enhanced the specific activities of sucrase and alkaline phosphatase in the duodenal brush border membrane af 20-day-old chick embryos. However, vitamin D₃ treatment increased alkaline phosphatase activity anly. Induction of alkaline phosphatase related to a change in fatty acid composition was thus caused by treatment with either hydrocortisone or vitamin D₃. Sucrase induction was not related to alteration of membrane lipids. These results suggest that hydrocortisone as well as vitamin D₃ might be involved in the developmental induction of membrane-bound enzyme (alkaline phosphatase) activity, affecting membrane fluidity through alteration of the membrane fatty acid composition.

収録刊行物

• 日本栄養・食糧学会誌

  日本栄養・食糧学会誌 43 (2), 133-138, 1990

  公益社団法人 日本栄養・食糧学会

キーワード

• fatty acid composition
• hydrocortisone
• vitamin D<SUB>3</SUB>
• sucrase
• alkaline phosphatase

詳細情報

• CRID: 1390282681268952704
• NII論文ID: 130000853215
• NII書誌ID: AN00311992
• DOI: 10.4327/jsnfs.43.133
• ISSN: 18832849
• ISSN: 02873516
• NDL書誌ID: 3671906
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3671906
• Web Site: https://search.jamas.or.jp/link/ui/1993131587
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可