Ｅｕｇｌｅｎａ　ｇｒａｃｉｌｉｓ　ＺにおけるプロビタミンＡの高速液体クロマトグラフによる定量および培養条件による変動

• 細谷 圭助

  和歌山大学教育学部家政教室

• 北岡 正三郎

  大阪府立大学農学部農芸化学科

書誌事項

タイトル別名

• Determination of provitamin A in Euglena gracilis Z by high performance liquid chromatography and changes of the contents under various culture conditions.

抄録

A method for determining provitamin A in Euglena gracilis Z was proposed by using high performance liquid chromatography (HPLC) and the culture conditions of E. gracilis for high yield of provitamin A were studied.<BR>1) Direct saponification of Euglena cells with 3.2 M KOH in methanol at 60°C for 20 min gave a satisfactory recovery of provitamin A.<BR>2) Saponified extract of Euglena cells was loaded onto a column packed with silicagel and eluted with different solvents. From the absorption maximum and retention time in HPLC the Euglena provitamin A was found to be consisted of β-carotene, echnenone and euglenanone.<BR>3) The content of β-carotene in the wild strain of E. gracilis grown with illumination was 577.8 μg/10⁹ cells. The wild strain grown in the dark and the bleached strain (a mutant derived from the wild strain) grown with or without illumination did not give higher contents.<BR>4) Among several carbon sources examined, glucose or malic acid gave high yield of β-carotene in culturing E. gracilis. The highest content of β-carotene was obtained by culturing Euglena in the Koren-Hutner medium containing glucose and malic acid as the major carbon sources.

収録刊行物

• 日本栄養・食糧学会誌

  日本栄養・食糧学会誌 37 (6), 519-524, 1984

  公益社団法人 日本栄養・食糧学会

詳細情報

• CRID: 1390001206293205888
• NII論文ID: 130000861485
• DOI: 10.4327/jsnfs.37.519
• ISSN: 18832849
• ISSN: 02873516
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可