Changes in ATPase Activities and Other Properties of Carp Myofibrillar Proteins during Ice-Storage

• SEKI Nobuo

  Laboratory of Biochemistry, Faculty of Fisheries, Hokkaido University

• NARITA Norihiro

  Laboratory of Biochemistry, Faculty of Fisheries, Hokkaido University

Bibliographic Information

Other Title

• コイ肉氷蔵中の筋原繊維たんぱく質のＡＴＰアーゼ活性およびその他の性状変化
• コイ肉氷蔵中の筋原繊維タンパク質のATPase活性およびその他の性状変化
• コイ ニク ヒョウゾウチュウ ノ キンゲン センイ タンパクシツ ノ ATPa

Abstract

Carp muscle was minced or chopped into small blocks and stored in ice for up to 16 days. Triton-washed myofibrils were prepared from the at-desath and stored muscles.<br> EDTA-ATPase activity of the myofibrils from the minced muscles decreased rapidly, while Ca²⁺- and Mg²⁺-ATPase activities decreased gradually during ice-storage with the exception that Mg²⁺-ATPase activity in the presence of EGTA increased. The increase in this ATPase activity was accompanied with a loss of Ca²⁺-sensitivity of the myofibrils. Little changes, however, occurred in ATPase activities of the myofibrils from the muscle blocks during ice-storage. SDS-polyacrylamide gel electrophoresis showed that the myofibrils from the fresh and stored muscles were similar in composition.<br> The change in Ca²⁺-ATPase activity of myosin B obtained from the stored muscles by extraction from the myofibrils paralleled that of the myofibrils during the storage. Mg²⁺-ATPase activity of the myofibrils from the fresh muscles was higher than that of the myosin B, but this difference reduced with increasing storage period of the muscles. Myosin B. obtained from the stored muscles contained aggregates more than the fresh myosin B. Myosin B aggregates sedimented by centrifugation at 20, 000×g for 20min had higher Mg²⁺-ATPase activity and lower EDTA-ATPase activity and lower Ca²⁺-sensitivity than myosin B in supernatant. The viscosity, ATP-sensitivity, and sedimentation behaviour of the myosin B were also measured.

Journal

• NIPPON SUISAN GAKKAISHI

  NIPPON SUISAN GAKKAISHI 46 (2), 207-213, 1980

  The Japanese Society of Fisheries Science

Keywords

• Aquatic Science

Details

• CRID: 1390001206412857344
• NII Article ID: 130000920543
• NII Book ID: AN00193422
• DOI: 10.2331/suisan.46.207
• ISSN: 1349998X
• ISSN: 00215392
• NDL BIB ID: 2170844
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010204007
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I2170844
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed