Immunohistochemical and Immunocytochemical Localization of Amylase in Rat Parotid Glands and von Ebner's Glands by Ion Etching-Immunoscanning Electron Microscopy
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- Yahiro Junko
- Department of Morphological Biology, Fukuoka Dental College
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- Inai Tetsuichiro
- Department of Morphological Biology, Fukuoka Dental College
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- Tsutsui Akihito
- Department of Preventive and Public Health Dentistry, Fukuoka Dental College
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- Sato Atsuko
- Department of Morphological Biology, Fukuoka Dental College
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- Nagato Toshikazu
- Department of Morphological Biology, Fukuoka Dental College
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- Taniguchi Kunihisa
- Department of Morphological Biology, Fukuoka Dental College
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- Tsuruga Eichi
- Department of Morphological Biology, Fukuoka Dental College
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- Sawa Yoshihiko
- Department of Morphological Biology, Fukuoka Dental College
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Abstract
The distribution of amylase in rat parotid glands and von Ebner’s glands was examined using ion etching-immunoscanning electron microscopy, which enables both light and electron microscopic observations of identical semi-thin resin sections immunolabeled with anti-α-amylase and immunogold in association with silver enhancement. At the light microscopic level, most acinar secretory granules (SG) and striated duct secretions of parotid glands were strongly stained dark brown. In von Ebner’s glands, acinar SG and duct secretions were weakly to strongly stained light to dark brown. At the electron microscopic level, labeling was observed as bright gold-silver particles. The labeling intensity of acinar SG of parotid glands was higher than that of von Ebner’s glands. In parotid glands, weak labeling of SG in transitional cells between acini and intercalated ducts, very weak labeling of SG in intercalated ducts, and strong labeling of striated duct secretions were observed. In von Ebner’s glands, the secretions and some SG of interlobular ducts were strongly labeled compared to those of intralobular ducts and SG of acini. Less amylase was synthesized in von Ebner’s acini compared to parotid acini, whereas von Ebner’s ducts may secrete significantly more amylase to modify saliva than parotid ducts.<br>
Journal
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- ACTA HISTOCHEMICA ET CYTOCHEMICA
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ACTA HISTOCHEMICA ET CYTOCHEMICA 44 (5), 201-212, 2011
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
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Details 詳細情報について
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- CRID
- 1390001204863426304
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- NII Article ID
- 130000941525
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- ISSN
- 13475800
- 00445991
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed