cDNA Cloning of Androgen-Repressed mRNA in Rat Seminal Vesicles: Partial Characterization of a cDNA Clone, pSvr-1.




    • Division of Physiology, Institute of Steroid Research, Tottori University School of Medicine


When the <I>in vitro</I> translation products of mRNAs from castrated animals (48h) were compared with those from androgen-treated animals (48 h) to survey the molecular mechanism of androgen-responsive gene expressions in the rat seminal vesicles, some peptide bands which were repressed in response to androgen were observed. From these findings, we constructed a partial cDNA library of poly (A+) RNAs which had been isolated from the seminal vesicles of castrated rats (48 h) and modestly enriched with respect to the concentration of androgen-repressed mRNAs by sucrose density gradient centrifugation, and screened by differential colony hybridization. One cDNA clone, pSvr-1, whose mRNA is markedly induced within 24h after castration of the animal in the seminal vesicles as well as in the ventral prostate, was isolated. pSvr-1 hybridized to a mRNA of 1, 700 nucleotides in length. Partial sequence analysis showed that this clone had highly homologous but not identical sequences to those reported for rat sulfated glycoprotein-2. This cDNA clone may provide a useful probe for the study of the negative regulation mechanism of gene expression by androgens.


  • Endocrinologia Japonica

    Endocrinologia Japonica 37(2), 233-238, 1990

    一般社団法人 日本内分泌学会