Lack of PTC Gene(ret Proto-oncogene Rearrangement) in Human Thyroid Tumors.
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- NAMBA HIROYUKI
- Department of Cell Physiology, Atomic Disease-Institute, Nagasaki University School of Medicine
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- YAMASHITA SHUNICHI
- Department of Cell Physiology, Atomic Disease-Institute, Nagasaki University School of Medicine
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- PEI HAI-CHENG
- The First Department of Internal Medicin
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- SHIKAWA NAOFUMI
- Department of Cell Physiology, Ito Hospital
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- VILLADOLID MARIA C.
- The First Department of Internal Medicin
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- TOMINAGA TAN
- The First Department of Internal Medicin
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- KIMURA HIRONORI
- The First Department of Internal Medicin
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- TSURUTA MASAKO
- The First Department of Internal Medicin
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- YOKOYAMA NAOKATA
- The First Department of Internal Medicin
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- IZUMI MOTOMORI
- The First Department of Internal Medicin
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- ISHIGAKI JITSUHIRO
- Ishigaki Thyroid Clinic
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- ITO KUNIHIKO
- Department of Cell Physiology, Ito Hospital
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- NAGATAKI SHIGENOBU
- The First Department of Internal Medicin
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Abstract
PTC gene, which is derived from the rearranged form of the ret proto-oncogene, was originally discovered in human thyroid papillary carcinomas. This gene has been thought to act as a tumorigenetic factor in thyroid carcinoma, although the action of PTC oncogene products is still unknown. To study the frequency of the PTC gene present in human thyroid carcinomas, we investigated four cell lines derived from thyroid carcinoma and 22 thyroid tumor tissue specimens. The reverse transcriptase-polymerase chain reaction (RT-PCR) method was performed to detect putative PTC mRNA. The presence of the PTC gene in genomic DNA was analyzed by Southern blot hybridization. PTC mRNA was detected by the RT-PCR method in only one papillary carcinoma cell line (TPC-1 cell). Southern gel analysis confirmed the rearrangement of the ret proto-oncogene in this cell line. In the other three cell lines and 22 tumor tissue specimens, however, neither the PTC gene or mRNA was detected. These results demonstrate that the prevalence of the PTC gene in thyroid tumor is low and may not be essential for human thyroid tumorigenesis. That our present results conflict with previous reports may be due to general differences in genetic background among races.
Journal
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- Endocrinologia Japonica
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Endocrinologia Japonica 38 (6), 627-632, 1991
The Japan Endocrine Society
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Details 詳細情報について
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- CRID
- 1390001206293738240
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- NII Article ID
- 130000952584
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- COI
- 1:CAS:528:DyaK3sXmsVKrtbs%3D
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- ISSN
- 21856370
- 00137219
- http://id.crossref.org/issn/00137219
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- PubMed
- 1823030
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed