Human Serum Thyroglobulin Determination Monoclonal Antibody One-step Assay

• KATO RYOJI

  Noguchi Thyroid Clinic and Hospital Foundation

• NOGUCHI SHIRO

  Noguchi Thyroid Clinic and Hospital Foundation

• NOGUCHI AKITO

  Noguchi Thyroid Clinic and Hospital Foundation

書誌事項

タイトル別名

• Minimum Interference of Autoantibody

抄録

Combination of two thyroglobulin monoclonal antibodies (monoAbs) recognizing epitopes which are rarely recognized by an antibody enabled us to develop a rapid one-step enzyme immunoassay of serum Tg. Of 87 monoclonal antibodies, 20 were selected for the purpose. The method is a sandwich technique employing a monoAb covering microplate and horse-radish peroxidase monoAb conjugate. A combination of monoAb 7A7A solid phase and 31A2E for the conjugate gave the best results. The assay takes60min and the minimal detectable amount is 2ng/ml. Intraassay variation is from 4 to 7%. Interassay variatoin is 5 to 12%. The recovery rate for Tg added to normal sera is between 89 and 111%.<BR>The correlation coefficient with the polyclonal antibody method in Tg hemagglutination negative sera is0.98.<BR>The presence of autoantibody in sera up to 10×2⁴hemagglutination titer does not affect the recovery rate to a statistically significant extent.

収録刊行物

• Ｅｎｄｏｃｒｉｎｏｌｏｇｉａ　Ｊａｐｏｎｉｃａ

  Ｅｎｄｏｃｒｉｎｏｌｏｇｉａ　Ｊａｐｏｎｉｃａ 34 (2), 171-178, 1987

  一般社団法人 日本内分泌学会

キーワード

• General Engineering
• Endocrinology

詳細情報

• CRID: 1390282681274408064
• NII論文ID: 130000953176
• DOI: 10.1507/endocrj1954.34.171
• COI: 1:CAS:528:DyaL2sXlsFSltL0%3D
• ISSN: 21856370
• ISSN: 00137219
• PubMed: 3622386
• Web Site: http://www.jstage.jst.go.jp/article/endocrj1954/34/2/34_2_171/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1989146493
• 本文言語コード: en
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  • PubMed
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