Effect of colchicine on the depletion and replenishment of cytoplasmic glucocorticoid receptor in rat liver after administration of glucocorticoid.

• ICHII SHOGO

  Division of Physiology, Institute of Steroid Research, and 1st Department of Internal Medicine

• YOSHIDA AKIO

  Tottori University School of Medicine

抄録

Pretreatment of rats with colchicine (3mg/kg body weight) modified the time course of depletion of the cytoplasmic binding sites for ³H-clexamethasone after administration of prednisolone (0.5 or 1.5mg/kg body weight). Colchicine also decreased the rate of the cytoplasmic receptor replenishment which was confirmed by application of this drug after completion of the cytoplasmic receptor translocation to nuclei (30 min after prednisolone injection). Addition of colchicine to the incubation mixture for in vitro binding of ³H-dexamethasonelabelled liver cytosol to isolated liver nuclei suspended in TKMS buffer (50 mM Tris-HCI, pH7.5, 50mM KCl, 5mM MgCl₂ and 250mM sucrose) evoked no measurable changes in the rate of the nuclear binding.

収録刊行物

• Ｅｎｄｏｃｒｉｎｏｌｏｇｉａ　Ｊａｐｏｎｉｃａ

  Ｅｎｄｏｃｒｉｎｏｌｏｇｉａ　Ｊａｐｏｎｉｃａ 32 (2), 225-231, 1985

  一般社団法人 日本内分泌学会

キーワード

• General Engineering
• Endocrinology

詳細情報

• CRID: 1390001206297379712
• NII論文ID: 130000953484
• DOI: 10.1507/endocrj1954.32.225
• ISSN: 21856370
• ISSN: 00137219
• PubMed: 4042987
• Web Site: http://www.jstage.jst.go.jp/article/endocrj1954/32/2/32_2_225/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可