Tissue calmodulin levels in normal and Graves' thyroids.

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  • INABA MASAAKI
    The Second Departments of Internal Medicine, Osaka City University Medical School
  • HAMADA NOBORU
    The Second Departments of Internal Medicine, Osaka City University Medical School
  • MORII HIROTOSHI
    The Second Departments of Internal Medicine, Osaka City University Medical School
  • NISHIZAWA YOSHIKI
    The Second Departments of Internal Medicine, Osaka City University Medical School
  • YAMAKAWA JUN-ICHI
    The Second Departments of Internal Medicine, Osaka City University Medical School
  • OHNO MARIKO
    The Second Departments of Internal Medicine, Osaka City University Medical School
  • TSUDA SACHIYO
    The Second Departments of Internal Medicine, Osaka City University Medical School
  • NO JAEDUK
    The Second Departments of Internal Medicine, Osaka City University Medical School
  • KANAYAMA YOSHIHARU
    The First Osaka City University Medical School
  • SAITOH HIFUMI
    Ito Hospital
  • MIMURA TAKASHI
    The First and Saiseikai Chuo Hospital
  • ITO KUNIHIKO
    Ito Hospital

抄録

Calmodulin levels in normal human thyroids and Graves' disease thyroids were measured by specific radioimmunoassay in the presence of ethyleneglycolbis-(β-aminoethyl ether)-N, N, N', N'-tetraacetic acid (EGTA). The calmodulin levels in tissues from patients with Graves' disease treated with thionamide drugs were significantly higher than those in normal tissues from euthyroid patients with solitary cold nodules (normal: 484±50ng/mg protein, mean±SE, n=15; Graves': 901±54ng/mg protein, n=48, p<0.001). Such a rise in calmodulin levels in Graves' disease thyroids was also present even after the administration of 50±g of T3 for 5 days before operation (828±137ng/mg protein, n=6, p<0.01).<BR>Calmodulin levels in Graves' disease thyroids were closely related to the cell height of follicular epithelium. Calmodulin levels in a columnar cell predominant group were significantly higher than those in a flat cell predominant or a cuboidal cell predominant group (columnar cell predominant: 1150±118ng/mg protein, n=13; flat cell predominant: 561±125ng/mg protein, n=3, p<0.05; cuboidal cell predominant: 596±40ng/mg protein, n=25, p<0.001).<BR>The increase in calmodulin content in Graves' disease thyroid could therefore possibly be attributed to the stimulation of the thyroid gland by the thyroid stimulating antibody. An immunofluorescence study demonstrated the presence of calmodulin immunoreactivity in the thyroid epithelial cells, particularly enriched in the apical border in the form of a granulated structure.

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