Simple method for collection of pancreatic islets by the use of Ficoll-Conray gradient.
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- OKEDA TOSHIMITSU
- The First Department of Medicine, Kyushu University Faculty of Medicine
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- ONO JUNKO
- The First Department of Medicine, Kyushu University Faculty of Medicine
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- TAKAKI RYOSABURO
- Department of Medicine, Medical College of Oita
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- TODO SATORU
- The First Department of Surgery, Kyushu University Faculty of Medicine
書誌事項
- タイトル別名
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- Simple Method for the Collection of Pancreatic Islets by the Use of Ficoll-Conray Gradient
- Simple Method for the Collection of Pan
この論文をさがす
抄録
Simple method for the collection of large numbers of viable pancreatic islets from normal rats is described. This method involves collagenase digestion of the pancreas, followed by the use of Ficoll-Conray discontinuous gradient for the separation of isolated islets from unwanted acinal debris.<BR>Ficoll-Conray A solution was prepared by mixing undialyzed 12.5% Ficoll and 33.4% Conray in the ratio 2: 1, to make a specific gravity of 1.095 and osmolarity of 396 mOsm/l. The solution B, C, and D, with specific gravities 1.084, 1.072, and 1.048, respectively were obtained by diluting A solution with distilled water.<BR>Using Ficoll-Conray gradient, about200viable islets which maintained excellently their morphology and function, were collected consistently from a young adult rat pancreas.
収録刊行物
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- Endocrinologia Japonica
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Endocrinologia Japonica 26 (4), 495-499, 1979
一般社団法人 日本内分泌学会
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詳細情報 詳細情報について
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- CRID
- 1390282681275410560
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- NII論文ID
- 130000954543
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- NII書誌ID
- AA00635052
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- COI
- 1:STN:280:Bi%2BD2cnltVA%3D
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- ISSN
- 21856370
- 00137219
- http://id.crossref.org/issn/00137219
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- NDL書誌ID
- 2092517
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- PubMed
- 387387
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可