Radioimmunoassay of Human Proinsulin C-peptide Using Synthetic Human Connecting Peptide
-
- KANEKO TOSHIO
- First Department of Internal Medicine, University of Tokyo Faculty of Medicine
-
- OKA HIROSHI
- First Department of Internal Medicine, University of Tokyo Faculty of Medicine
-
- MUNEMURA MASAHIDE
- First Department of Internal Medicine, University of Tokyo Faculty of Medicine
-
- ODA TOSHITSUGU
- First Department of Internal Medicine, University of Tokyo Faculty of Medicine
-
- YAMASHITA KAMEJIRO
- First Department of Internal Medicine, University of Tokyo Faculty of Medicine
-
- SUZUKI SEIJI
- First Department of Internal Medicine, University of Tokyo Faculty of Medicine
-
- YANAIHARA NOBORU
- First Department of Internal Medicine, University of Tokyo Faculty of Medicine
-
- HASHIMOTO TADASHI
- First Department of Internal Medicine, University of Tokyo Faculty of Medicine
-
- YANAIHARA CHIZUKO
- Department of Bioorganic Chemistry, Shizuoka College of Pharmacy
この論文をさがす
抄録
Radioimmunoassay of human C-peptide was established using antiserum to synthetic human connecting peptide and synthetic tyrosylated human connecting peptide. Synthetic human connecting peptide and natural human C-peptide showed the same degree of cross-reactivity with the synthetic human connecting peptide antiserum. Natural human proinsulin reacted approximately one fourth as well as the human connecting peptide or natural human C-peptide when expressed on an equimolar basis.<BR>Synthetic porcine and bovine connecting peptide, human insulin and natural porcine proinsulin did not react with the human connecting peptide antiserum. Synthetic human connecting peptide did not cross with insulin antiserum. The senstitivity of the radioimmunoassay was 0.05ng/tube.<BR>The assay system was available to determine C-peptide immunoreactivity in plasma, because proinsulin and proinsulin-like components had been reported to be very low in plasma. The fasting level of C-peptide immunoreactivity in plasma of normal subjects assayed by this system was 0.88±0.21ng/ml.
収録刊行物
-
- Endocrinologia Japonica
-
Endocrinologia Japonica 21 (2), 141-145, 1974
一般社団法人 日本内分泌学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681274058240
-
- NII論文ID
- 130000956561
-
- NII書誌ID
- AA00635052
-
- COI
- 1:CAS:528:DyaE2MXltlKrtg%3D%3D
-
- ISSN
- 21856370
- 00137219
-
- NDL書誌ID
- 7672951
-
- PubMed
- 4408341
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可