Epidemiological Survey for Brucella in Wildlife and Stray Dogs, a Cat and Rodents Captured on Farms

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<i>Brucella</i> infections in wildlife originate either from contact with infected livestock or from a natural sustainable reservoir in wildlife populations. As South Korea has set a goal of brucellosis eradication by 2013, it is necessary to determine the prevalence of <i>Brucella</i> in wildlife and wild rodents. This information will play an important role in the control of brucellosis. Because of the absence of prominent clinical signs, direct and indirect laboratory tests are essential for diagnosing brucellosis. In this study, tissue and blood samples were taken from wild animals, abandoned dogs, a cat and wild rodents, and they were tested for <i>Brucella</i> or <i>Brucella</i>-specific antibodies by isolation, PCR and serology. Results showed that 18.6% (33/177) of blood samples were positive by PCR, and 5.7% (11/194) were positive by C-ELISA. However, none of these samples yielded culturable bacteria. Of the tissue samples, 9.7% (8/82) were positive by PCR. <i>Brucella</i> was isolated from only one tissue culture from a Chinese water deer carcass. This <i>Brucella</i> species was identified as <i>Brucella abortus</i> biovar 1 by biotyping, 16S rRNA PCR and the Bruce-ladder PCR assay. In this study, we reported the prevalence of <i>Brucella</i> in wildlife, dogs, a cat and rodents by using serological and molecular methods, and we report the first isolation of <i>B. abortus</i> in wild Chinese water deer in South Korea.<br>


  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 73(12), 1597-1601, 2011



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