Application of Truncated Nucleocapsid Protein (N) for Serotyping ELISA of Murinae-Associated Hantavirus Infection in Rats

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  • YASUDA Shumpei P.
    Department of Microbiology, Hokkaido University Graduate School of Medicine
  • YOSHIMATSU Kumiko
    Department of Microbiology, Hokkaido University Graduate School of Medicine
  • KOMA Takaaki
    Department of Microbiology, Hokkaido University Graduate School of Medicine
  • SHIMIZU Kenta
    Department of Microbiology, Hokkaido University Graduate School of Medicine
  • ENDO Rika
    Department of Microbiology, Hokkaido University Graduate School of Medicine
  • ISOZUMI Rie
    Department of Microbiology, Hokkaido University Graduate School of Medicine
  • ARIKAWA Jiro
    Department of Microbiology, Hokkaido University Graduate School of Medicine

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Abstract

Truncated recombinant nucleocapsid proteins (trNs) that lack N-terminally located cross-reactive epitopes of four Murinae rodent-associated hantaviruses, Seoul virus (SEOV), Thailand virus, Hantaan virus (HTNV) and Dobrava-Belgrade virus, were produced by using a baculovirus expression system. ELISA with the trNs as antigens enabled serotyping of immune sera from rats experimentally inoculated with the corresponding hantaviruses with cut-off OD values of 60% of those of whole N of HTNV. The trN-based ELISA could serotype 12 out of 13 sera obtained from wild rodents (Rattus norvegicus) naturally infected with SEOV using the 60% cut-off value. These results indicate that screening with whole N followed by serotyping with trNs using a cut-off OD value of 60% of that of whole N is a useful method for serological surveillance of Murinae-associated hantavirus infection among rodents.<br>

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