Lectin Binding Pattern of Gastric Mucosa of Pacific White-Sided Dolphin, Lagenorhynchus obliquidens

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  • SHIMOKAWA Tetsuya
    Department of Anatomy and Embryology, Ehime University Graduate School of Medicine
  • DOIHARA Takuya
    Department of Anatomy and Embryology, Ehime University Graduate School of Medicine
  • MAKARA Manami
    Division of Vertebrates, Department of Zoology, National Museum of Nature and Science
  • MIYAWAKI Kyoji
    Shikoku-Chuoh Medical and Welfare General College
  • NABEKA Hiroaki
    Department of Anatomy and Embryology, Ehime University Graduate School of Medicine
  • WAKISAKA Hiroyuki
    Department of Liberal Arts, Ehime Prefectural University of Health Sciences
  • KOBAYASHI Naoto
    Medical Education Center, Ehime University Graduate School of Medicine
  • MATSUDA Seiji
    Department of Anatomy and Embryology, Ehime University Graduate School of Medicine

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Abstract

The stomach of the Pacific white-sided dolphin is divided into three parts: forestomach, proper gastric gland portion, and pyloric chamber. The histological features of the dolphin stomach are similar to those of terrestrial mammal stomachs, although the distribution of glycoconjugates in mucosal cells of the dolphin stomach is unknown. To learn about glycoconjugates in cetacean gastric mucosa, the glycoconjugate distribution in the mucous epithelium of the Pacific white-sided dolphin was studied using 21 lectins. Among the lectins tested, GSL-I and DBA specifically labelled the superficial layer of the forestomach epithelium. GSL-I, SBA, RCA-I, VVA, GSL-II, DSL, LEL, STL, s-WGA, WGA, PNA, and Jacalin labelled the luminal surface of the chief cells in the proper gastric gland. GSL-I, SBA, RCA-I, DSL, LEL, STL, s-WGA, PNA, and LCA labelled tubular structures in the cytoplasm of parietal cells. The surface portion of the pits in the pyloric chamber strongly reacted with RCA-I, GSL-II, WGA, PNA, LCA, PHA-L, and UEA-I, whereas the neck portion reacted weakly. Although lining one tubular portion, individual secretory cells in the pyloric gland displayed a heterogeneous reaction. This is the first report on the lectin histochemistry of a cetacean stomach and reveals GSL-I and DBA as specific marker lectins for the cornified stratified squamous epithelium cells of the Pacific white-sided dolphin. The stomachs of cetaceans and terrestrial mammals have similar histological features and mucous glycoconjugate content.<br>

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