消化管粘膜におけるアルゴンレーザー光励起けい光に対する組織内ヘモグロビンの影響  [in Japanese] The influence of tissue hemoglobin on Argon laser induced fluorescence at gastrointestinal mucosa.  [in Japanese]

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Abstract

胃及び大腸の正常粘膜面におけるアルゴンレーザー光励起蛍光スペクトルは540及び580nm付近に陥凹があり, 560及び600nm付近が突出している. この520nmから600nmまでの凹凸はエピネフリン液の散布や測定用ファイバーによる対象粘膜の圧迫により消失あるいは減少する. 蛍光観察用スライドグラスにて作製した chamber 内にヘモグロビン溶液をいれ, 切除胃固定標本の粘膜筋板を露出させた部位に置いて記録した蛍光スペクトルは生体内の粘膜面から得られるものと酷似している. 従つて, 540及び580nm付近の蛍光強度の減弱と560及び600nm付近の相対的増強は, 組織内ヘモグロビンによつて蛍光が吸収されて生じるものと考えられる.

The intensity of Argon laser induced fluorescence at the normal mucosa of the gastrointertinal tract is relatively weak at about 540 and 580nm, and strong at about 560 and 600nm. This mechanism is unknown. We report its mechanism and the possibility of its clinical application.<br>By spraying epinephrine solution to the mucosa or strongly pushing the optical quartz fiber bundles for measurement against the mucosa, the intensity of the fluorescence increased and its spectrum showed a smooth declined slope probably due to no absorption of the fluorescence by blood in the tissue.<br>We made a chamber of two pieces of slide glass and placed it on the fixed resected specimen of the stomach, in which the mucosal layer was removed and the muscularis mucosa was exposed. When the chamber was filled with distilled water, Argon laser induced fluorescence spectrum on it showed a smooth curve. But when it was filled with hemoglobin solution, the spectrum pattern was similar to that of the normal mucosa.<br>The partial absorption of the Argon laser induced fluorescence by hemoglobin resulted in the irregular fluorescence spectrum curve such as troughs at about 540 and 580nm and peaks at about 560 and 600nm.

Journal

  • Nippon Shokakibyo Gakkai Zasshi

    Nippon Shokakibyo Gakkai Zasshi 82(8), 1853-1857, 1985

    The Japanese Society of Gastroenterology

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