浸透圧ストレスによるニンジン不定胚形成の誘導

書誌事項

タイトル別名
  • Induction of Somatic Embryogenesis in Carrot by Osmotic Stress.
  • 浸透圧ストレスによるニンジン不定胚形成の誘導〔英文〕
  • シントウアツ ストレス ニ ヨル ニンジン フテイ ハイ ケイセイ ノ ユウド

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抄録

Segments of cotyledon and apical tip taken from 1-week-old seedlings of Daucus carota L. were cultured for 3 weeks on hormone-free Murashige and Skoog's (MS) medium containing high amounts of sucrose (0.3-0.7M) and then transferred to the same medium with lower sucrose concentration (0.09M). Somatic embryos were formed from the segments 3 to 6 weeks after the transfer. On the other hand, hypocotyl segments given the same treatment did not produce somatic embryos. When the primary culture medium contained 0.61M mannitol and 0.09M sucrose, cotyledon segments and apical tip segments also produced somatic embryos when transferred to hormone-free MS medium with 0.09M sucrose. In all cases, somatic embryos were formed directly from the explants without visible callus formation.

収録刊行物

  • 植物組織培養

    植物組織培養 10 (1), 38-44, 1993

    日本植物細胞分子生物学会

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