Ｉｎ　ｓｉｔｕ　ｈｙｂｒｉｄｉｚａｔｉｏｎ法による肝組織内ＨＢＶ　ＤＮＡの検出　　各種ＨＢＶマーカーとの関連

• 道尭 浩二郎

  愛媛大学医学部第3内科

書誌事項

タイトル別名

• Detection of HBV DNA by an in situ hybridization method. Its distribution in relation to other HBV markers.
• its distribution in relation to other HBV markers
• 各種HBVマーカーとの関連

抄録

Hepatitis B virus (HBV) DNA in hepatocytes was visualized by an in situ hybridization method using ³H-labelled probe. HBV DNA was visualized as silver grains on 47 of 63 liver specimens from patients with chronic hepatitis B. The distribution of HBV DNA positive hepatocytes was classified into three patterns: diffuse (HBV DNA distributed diffusely within the section), lobular (HBV DAN was present in 1/3-2/3 of the section) and spotty. Nineteen of 47 specimens showed diffuse, 17 showed lobular and 11 showed spotty pattern. Most cases of diffuse and lobular pattern were serum HBeAg-positive and HBV DNA polymerase activity-positive. Immunohistochemical study of HBsAg and HBcAg in serial sections revealed that HBcAg positive hepatocytes were usually HBV DNA rich, although HBsAg positive hepatocytes were not always HBV DNA rich. These data indicate that HBV DNA detected by in situ hybridization is a useful marker for HBV replication.

収録刊行物

• 日本消化器病学会雑誌

  日本消化器病学会雑誌 85 (6), 1260-1268, 1988

  一般財団法人 日本消化器病学会

キーワード

• B型慢性肝障害
• in situ hybridization 法
• HBe抗原
• HBc抗原

詳細情報

• CRID: 1390001206396464000
• NII論文ID: 130001066644
• DOI: 10.11405/nisshoshi1964.85.1260
• ISSN: 13497693
• ISSN: 04466586
• PubMed: 3225944
• Web Site: https://search.jamas.or.jp/link/ui/1989197241
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可