書誌事項
- タイトル別名
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- Characterization of cholecystokinin receptors in gastric chief cells - Effect of CCK analogs and CCK receptor antagonists on chief cells.
- Effect of CCK analogs and CCK receptor antagonists on chief cells
- -第1報-CCK類似体及び各種CCK受容体拮抗剤のペプシノーゲン分泌反応及び主細胞内Ca<sup>2+</sup>濃度に及ぼす効果
抄録
In isolated guinea pig gastric chief cells, gatrin-I and cholecystokinin-hexapeptide (CCK-4) stimulated pepsinogen release. However, the efficacies of these two peptide were 51% of that observed with CCK-octapeptide (CCK8). CR1409 and L-364718, both of which are new CCK receptor antagonists in pancreatic acinar cells, also inhibited 10-8M CCK8-stimulated pepsinogen release with a half-maximal inhibitory concentration (IC50) observed at 3×10-9M, respectively. The dose response curve to CCK8 for pepsinogen release shifted to the right in the presence of CR1409 or, L-364718. The IC50 of these two antagonists for the CCK8-stimulated increase in cytosolic Ca2+ concentration monitored by Fura-2 were equal to those for CCK8-stimulated pepsinogen release. By contrast, the IC50 of dibutyryl cyclic GMP, a well-known CCK receptor antagonist, for CCK8-stimulated pepsinogen release was less than that for CCK8-stimulated increase in cytosolic Ca2+ concentration. Results suggested that CCK receptors in gastric chief cells are unique and may be different from CCK receptors in other tissues previously repoted.
収録刊行物
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- 日本消化器病学会雑誌
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日本消化器病学会雑誌 86 (7), 1424-1428, 1989
一般財団法人 日本消化器病学会