サトイモ(Colocasia esulenta Schott)カルスからの植物体の再生

書誌事項

タイトル別名
  • Plantlet Regeneration from Taro (Colocasia esculenta Schott) Callus.
  • サトイモ(Colocasia esculenta Schott)カルスからの植物体の再生〔英文〕
  • サトイモ Colocasia esculenta Schott カルス カラ

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抄録

Taro (Colocasia esculenta) callus was induced from shoot tip explants on a modified Nitsch medium supplemented with 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 6-benzyladenine (BA) at 1 mg•liter-1 each. The effect of agar concentration (0, 0.2, 0.4, 0.6, 0.8%, W/V) in a modified Murashige and Skoog (MS) medium on the regeneration of shoots from taro callus was investigated. Shoot differentiation was observed only in 0.2, 0.4 and 0.6% agar concentrations. A high level of shoot differentiation at 64% was measured on 0.6% agar medium. However, root differentiation was observed regardless of agar concentrations.<BR>Shoot differentiation was observed on the medium supplemented with 1 mg•liter-1 BA as early as 10 weeks of culture.<BR>Addition of 0.1% (W/V) charcoal did not directly induce the regeneration of shoots from the callus, but lengthened the longetivity of the calli since no necrosis was observed after 20 weeks of culture.<BR>Proliferation of regenerated shoots was enhanced with the addition of naphthalene acetic acid (NAA) and BA at 1 mg•liter-1 each. The plantlets can be readily potted in a vermiculite medium for acclimatization before planting in the field.

収録刊行物

  • 園芸学会雑誌

    園芸学会雑誌 60 (4), 935-940, 1992

    一般社団法人 園芸学会

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