Turnover Ability of a Designed RNA Acting as a Template for Chemical Peptide Ligation

• YAMASHITA Kohei

  Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University

• KASHIWAGI Norimasa

  Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University

• FURUTA Hiroyuki

  Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University International Research Center for Molecular Systems, Kyushu University

• IKAWA Yoshiya

  Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University International Research Center for Molecular Systems, Kyushu University

Abstract

A designed self-folding RNA possessing two peptide-recognition motifs served as a template for the chemical ligation of two RNA-binding peptides under stoichiometric conditions. In this study, we investigated the turnover ability of this template RNA in facilitation of peptide ligation and found that the RNA exhibited modest turnover ability under conditions in which its 3D structure was marginally stable.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 75 (10), 2021-2024, 2011

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• RNA
• native chemical ligation
• Rev-peptide
• N-peptide
• P4–P6 RNA

Details

• CRID: 1390282681454666368
• NII Article ID: 130001257631
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.110255
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 11286662
• Web Site: http://id.ndl.go.jp/bib/11286662
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11286662
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110255
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed