Immunohistochemical Localization of the Water Channels AQP4 and AQP5 in the Rat Pituitary Gland
-
- Matsuzaki Toshiyuki
- Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine
-
- Inahata Yuki
- Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School Department of Biology, Division of Natural Sciences, International Christian University
-
- Sawai Nobuhiko
- Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School
-
- Yang Chun-Ying
- Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School
-
- Kobayashi Makito
- Department of Biology, Division of Natural Sciences, International Christian University
-
- Takata Kuniaki
- Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine
-
- Ozawa Hitoshi
- Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School
Search this article
Abstract
The pituitary gland is composed of the adenohypophysis and neurohypophysis. The adenohypophysis contains endocrine cells, folliculo-stellate (FS) cells, and marginal layer cells, whereas the neurohypophysis mainly comprises axons and pituicytes. To understand the molecular nature of water transfer in the pituitary gland, we examined the immunohistochemical localization of the membrane water channels aquaporin-4 (AQP4) and AQP5 in rat tissue. Double immunofluorescence analysis of AQP4 and S100 protein, a known marker for FS cells, marginal layer cells, and pituicytes, clearly revealed that FS cells and marginal layer cells in the adenohypophysis and the pituicytes in pars nervosa are positive for AQP4. AQP5 was found to be localized at the apical membrane in some marginal layer cells surrounding the Rathke’s residual pouch, in which AQP4 was observed to be localized on the basolateral membranes. These results suggest the following possibilities: 1) FS cells especially require water for their functions and 2) transepithelial water transfer could occur between the lumen of Rathke’s residual pouch and the interstitial fluid in the adenohypophysis through the AQP4 and AQP5 channels in the marginal layer cells.<br>
Journal
-
- ACTA HISTOCHEMICA ET CYTOCHEMICA
-
ACTA HISTOCHEMICA ET CYTOCHEMICA 44 (6), 259-266, 2011
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY