A Simple Enzymatic Quantitative Analysis of Triglycerides in Tissues.

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Author(s)

    • DANNO Hiroshi
    • Laboratory of Nutrition, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
    • JINCHO Yuu
    • Laboratory of Nutrition, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
    • BUDIYANTO Slamet
    • Laboratory of Nutrition, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
    • FURUKAWA Yuji
    • Laboratory of Nutrition, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
    • KIMURA Shuichi
    • Laboratory of Nutrition, Department of Food Chemistry, Faculty of Agriculture, Tohoku University

Abstract

We determined a method to measure the triglyceride levels in tissues by using a modified enzymatic kit. This enzymatic kit was originally designed to be used to measure the triglyceride levels in plasma. Our method of triglyceride level determination includes dissolving the tissue lipid extracts in an alcohol. Before using the enzymatic kit directly, the lipids were dissolved in tent-butyl alcohol, then a Triton X-100/methyl alcohol mixture was added (1/1 by volume). The presence of organic surfactants such as tent-butyl alcohol and methyl alcohol, and of a surfactant such as Triton X-100, did not interfere with the enzymatic activity. This method enabled us to determine triglyceride levels between 10 and 90 nmol, by using a spectrophotometer to measure the absorbances.

Journal

  • Journal of Nutritional Science and Vitaminology

    Journal of Nutritional Science and Vitaminology 38(5), 517-521, 1992

    Center for Academic Publications Japan

Cited by:  1

Codes

  • NII Article ID (NAID)
    130001369472
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0301-4800
  • Data Source
    CJPref  J-STAGE 
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