Analysis of the Comprehensive Effects of Polyunsaturated Fatty Acid on mRNA Expression Using a Gene Chip

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  • FUJIWARA Yoko
    Department of Food Science and Nutrition, Ochanomizu University Division of Clinical Nutrition, National Institute of Nutrition and Health
  • YOKOYAMA Masayo
    Department of Food Science and Nutrition, Ochanomizu University
  • SAWADA Rumi
    Department of Food Science and Nutrition, Ochanomizu University
  • SEYAMA Yousuke
    Department of Food Science and Nutrition, Ochanomizu University
  • ISHII Masami
    Genome Sciences, Research Center for Advanced Science and Technology
  • TSUTSUMI Shunichi
    Genome Sciences, Research Center for Advanced Science and Technology
  • ABURATANI Hiroyuki
    Genome Sciences, Research Center for Advanced Science and Technology
  • HANAKA Satoko
    Division of Clinical Nutrition, National Institute of Nutrition and Health Medical School of Teikyo University
  • ITAKURA Hiroshige
    Division of Clinical Nutrition, National Institute of Nutrition and Health Department of Life Science, Ibaraki Christian University
  • MATSUMOTO Akiyo
    Division of Clinical Nutrition, National Institute of Nutrition and Health Department of Clinical Dietetics and Human Nutrition

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Abstract

To investigate the comprehensive effects of polyunsaturated fatty acids (PUFA) on gene expression, we analyzed changes of mRNA expression in PUFA-treated HepG2 cells using a DNA micro array. We incubated HepG2 cells for 24h with or without 0.25mM oleic acid (OA), arachidonic acid (AA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA), and then compared the expression profiles of thousands of genes using a GeneChip. PUPA influenced the expression of various genes related to cell proliferation, growth and adhesion, as well as for many transcription factors including sterol regulatory element binding proteins (SREBP). Treatments with AA, EPA, and DHA repressed the expression of genes related to cholesterol and lipid metabolism. Moreover, data from gene chip analysis proved that PUFA reduced the expression of prostasin, which is a serine protease. By measuring the mRNA levels of SREBPs, mevalonate pyrophosphatase and prostasin using quantitative RT PCR, we confirmed the effect of PUFA revealed by gene chip analysis. These data might provide useful clues with which to explore novel functions of PUFA.

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