Inhibition of Lipase Activities by Citrus Pectin

DOI Web Site 被引用文献5件 参考文献25件
  • TSUJITA Takahiro
    Division of Medical Bioscience, Department of Bioscience, Integrated Center for Sciences, Ehime University
  • SUMIYOSHI Maho
    Division of Medical Bioscience, Department of Bioscience, Integrated Center for Sciences, Ehime University
  • HAN Li-Kun
    Division of Food and Health Environment, Faculty of Environmental and Symbiotic Science, Prefectural University of Kumamoto
  • FUJIWARA Tsutomu
    Research Laboratory, Nihonshokken Ltd.
  • TSUJITA Junji
    Research Laboratory, Nihonshokken Ltd.
  • OKUDA Hiromichi
    Division of Food and Health Environment, Faculty of Environmental and Symbiotic Science, Prefectural University of Kumamoto

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The oral administration of pectin to rats reduced and delayed the peak plasma triacylglycerol concentration. Pectin inhibited the hydrolysis of trioleoylglycerol emulsified with soybean phosphatidylcholine by pancreatic, carboxylester, and lingual lipases in a concentration-dependent manner. However, the effective concentration of pectin for lingual lipase was 100 times lower than that for pancreatic lipase. Pectin did not inhibit the tributyrin- and p-nitrophenylbutyrate-hydrolyzing activities by pancreatic and carboxylester lipase. When low molecular weight pectin was assayed, pectin at a molecular weight of 90, 000 (MW 90) most strongly inhibited three lipase activities. When the effect of pH on pectin inhibition was analyzed using pancreatic lipase, strong inhibition was observed at an acidic pH (below pH 7.0). In the assay system, the pancreatic lipase protein levels in the supernatant and fat layer were estimated by Western blotting with an anti-pancreatic lipase antibody. Pectin reduced the amount of pancreatic lipase protein in the fat layer in a concentration-dependent manner and concomitantly increased that in the supernatant. These results suggest that pectin may interact with emulsified substrates and inhibit the adsorption of lipase to the surface of substrate emulsion.

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