ブドウ球菌コアグラーゼの精製と性状に関する研究

• 一言 広

  東京都立衛生研究所細菌部

書誌事項

タイトル別名

• Studies on the Purification of Staphylococcal Coagulase and Its Properties
• ブドウ キュウキン コアグラーゼ ノ セイセイ ト セイジョウ ニ カンスル ケンキュウ

抄録

A purified coagulase was obtained from a culture filtrate of Staphylococcus aureus St-213 which proved to be homogenous in immunological, electrophoretical and Spinco Model E ultracentrifugal studies. Treatment of the material with trichloroacetic acid prior to passing through Dowex-1 and Diethylaminoethyl-Sephadex A-50 columns yielded a product which showed a 280 fold increase in activity per mg of nitrogen than the crude culture filtrate. The sedimentation coefficient of the treated material was S_{20, w}=1.59S.<br>The treated material was composed of 9 amino acids (i.e. lysin, aspartate, threonine, serine, glutamine, glycine, alanine, valine and methionine) and lacked carbohydrates, lipids and phosphates. Therefore, this purified material may be classified as a purified protein derivative (PPD) capable of exhibiting a specific coagulase type reaction against homologous and heterologous type antiserum when tested immunologically using the agar gel diffusion technique.

収録刊行物

• 日本細菌学雑誌

  日本細菌学雑誌 20 (11), 627-633, 1965

  日本細菌学会

キーワード

• General Medicine

詳細情報

• CRID: 1390282681432279424
• NII論文ID: 130001382265
• NII書誌ID: AN00189800
• DOI: 10.3412/jsb.20.627
• ISSN: 18824110
• ISSN: 00214930
• NDL書誌ID: 8381044
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8381044
• Web Site: https://search.jamas.or.jp/link/ui/1966009703
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可