High Prevalence of <i>qnr</i> and <i>aac(6′)-Ib-cr</i> Genes in Both Water-Borne Environmental Bacteria and Clinical Isolates of <i>Citrobacter freundii</i> in China

• Zhang Rong

  Second affiliated hospital of Zhejiang University, Zhejiang University Graduate School of Pharmaceutical Sciences, Osaka University

• Ichijo Tomoaki

  Graduate School of Pharmaceutical Sciences, Osaka University

• Huang Yong-Lu

  Second affiliated hospital of Zhejiang University, Zhejiang University

• Cai Jia-Chang

  Second affiliated hospital of Zhejiang University, Zhejiang University

• Zhou Hong-Wei

  Second affiliated hospital of Zhejiang University, Zhejiang University

• Yamaguchi Nobuyasu

  Graduate School of Pharmaceutical Sciences, Osaka University

• Nasu Masao

  Graduate School of Pharmaceutical Sciences, Osaka University

• Chen Gong-Xiang

  Second affiliated hospital of Zhejiang University, Zhejiang University

Bibliographic Information

Other Title

• High Prevalence of qnr and aac(6')-Ib-cr Genes in Both Water-Borne Environmental Bacteria and Clinical Isolates of Citrobacter freundii in China
• High prevalence of qnr and aac(6′)-Ib-cr genes in both water-borne environmental bacteria and clinical isolates of Citrobacter freundii in China

Abstract

We investigated the prevalence of qnr and aac(6′)-Ib-cr genes in water-borne environmental bacteria and in clinical isolates of Enterobacteriaceae, as well as the subtypes of qnr. Environmental bacteria were isolated from surface water samples obtained from 10 different locations in Hangzhou City, and clinical isolates of Citrobacter freundii were isolated from several hospitals in four cities in China. qnrA, qnrB, qnrS, and aac(6′)-Ib-cr genes were screened using PCR, and the genotypes were analyzed by DNA sequencing. Ten of the 78 Gram-negative bacilli isolated from water samples were C. freundii and 80% of these isolates carried the qnrB gene. qnrS1 and aac(6′)-Ib-cr genes were detected in two Escherichia coli isolates and qnrS2 was detected in one species, Aeromonas punctata. The qnr and aac(6′)-Ib-cr genes were present in 75 (72.8%) and 12 (11.6%) of 103 clinical isolates of C. freundii, respectively. Of the clinical C. freundii isolates with the qnr gene, 65 isolates (63.1%) carried qnrB, but only three (2.9%) and one (1.0%) carried qnrA1 and qnrS2, respectively, while five isolates carried both qnrA1 and qnrB, and one isolate carried both qnrS1 and qnrB. The qnrB9 gene was the dominant qnrB subtype, followed by qnrB8 and qnrB6. Southern hybridization studies indicated that the qnr genes are located on different plasmids. Plasmids isolated from both environmental and clinical C. freundii isolates appeared to be homogenous.<br>

Journal

• Microbes and Environments

  Microbes and Environments 27 (2), 158-163, 2012

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• <i>Enterobacteriaceae</i>
• environmental bacteria
• quinolone resistance gene

Details

• CRID: 1390001204344537600
• NII Article ID: 10030431898
• NII Article ID: 130001396931
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me11308
• COI: 1:STN:280:DC%2BC38jps1KgtQ%3D%3D
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 023727709
• PubMed: 22791048
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023727709
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/27/2/27_ME11308/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed