MOLECULAR EPIDEMIOLOGICAL ANALYSIS OF MYCOBACTERIUM KANSASII ISOLATES
-
- YOSHIDA Shiomi
- Clinical Research Center
-
- SUZUKI Katsuhiro
- Clinical Research Center
-
- TSUYUGUCHI Kazunari
- Clinical Research Center
-
- IWAMOTO Tomotada
- Kobe Institute of Health
-
- OKADA Masagi
- Clinical Research Center
-
- SAKATANI Mitsunori
- Departm ent of Respiratory Medicine, National Hospital Organization Kinki-chuo Chest Medical Center
Bibliographic Information
- Other Title
-
- Mycobacterium kansasii株における分子疫学的解明
- Mycobacterium kansasiiカブ ニ オケル ブンシエキガクテキ カイメイ
Search this article
Abstract
[Purpose] To make molecular epidemiological analysis of Mycobacterium k ansasii (Al, k ansasii) isolates.<BR>[Methods] We examined 174 M. kansasii is olates from clinical samples of patients at National Hospital Organization Kinki-chuo Chest Medical Center from June 1, 2002 to August 31, 2005 by polymerase chain reaction (PCR) -restriction analysis (PRA) of the heat shock protein (hsp) 65 gene (hsp 65PRA), sequencing (ITS, 16S-23S internal transcribed spacer, and hsp 65 for discrepant case between hsp 65-PRA and ITS sequence), pulsed-field gel electrophoresis (PFGE), and restriction fragment length polymorphism (RFLP) with the major polymorphic tandem repeat (MPTR) probe and the IS 1652probe of genomic DNA.<BR>[Results] Of the 174 M. kansasii isolates, 170 strains were classified as M. kansasii type I using hsp 65-PRA, while two isolates belonged to type II and one each isolate to type II b and VI, respectively. Although the ITS sequence of these isolates also identified the same region of polymorphism by hsp65-PRA, only type II b might be revealed atypical type II, a transitional type from typical type II to intermediate type I by hsp 65 sequence. The polymorphic patterns by RFLPs with MPTR and 1S1652 probe were shown specific for each homogeneous cluster by hsp 65-PRA. In addition, 159 isolates were recognized the same common pattern A by PFGE analysis. In contrast, the rest 15 isolates revealed significant polymorphism within 11 isolates of type I, and 4 isolates among type II, Ub, and VI.<BR>[Discussion] W e verified the M. kansasii genotype I was predominant, with the same pattern of major worldwide type regions, and reflected a very tight clonal structure. Type I was furthermore indicated recognition of subtypes by PFGE analysis.
Journal
-
- Kekkaku(Tuberculosis)
-
Kekkaku(Tuberculosis) 82 (2), 103-110, 2007
JAPANESE SOCIETY FOR TUBERCULOSIS
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001205000618880
-
- NII Article ID
- 130001428405
-
- NII Book ID
- AN00073442
-
- COI
- 1:CAS:528:DC%2BD2sXjsV2hsLc%3D
-
- ISSN
- 18842410
- 00229776
-
- NDL BIB ID
- 8689083
-
- Text Lang
- ja
-
- Data Source
-
- JaLC
- NDL
- CiNii Articles
-
- Abstract License Flag
- Disallowed