サケ科魚類せっそう病原因菌Aeromonas salmonicidaの産生する病因物質について III  Aeromonas salmonicida Ar‐4 (EFLD)の産生するプロテアーゼの特性 Enzymatic properties of the purified extracellular protease of <i>Aeromonas salmonicida</i>, Ar-4 (EFDL)

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In a previous paper, we examined the toxicity of the extracellular protease of <i>A. salmonicida</i>, Ar-4 (EFDL) on yamabe (<i>Oncorhynchus masou f. ishikawai</i>) and goldfish (<i>Carassius auratus</i>). From these results, we considered the protease secreted by this bacterium was a causative agent of furunculosis.<br> In this paper, we observed enzymatic properties of the purified protease. The results obtained were summarized as follows:<br> 1. The molecular weight of purified protease was estimated to be 71, 000 by sodium dodecyl sulfate polyacrylamide gel electrophoresis.<br> 2. The purified protease showed maximal activity at pH 9.4 and 50°C. It was stable over the pH range 5.0 to 10.0 and was completely inactivated by temperature at 56°C.<br> 3. The protease was presumably classified an alkaline serine protease and it showed chymotryptic properties since it was significantly inhibited by diisopropylfluorophosphate (DFP) and tosyl-phenylalanine-chloromethyl ketone (TPCK) and hydrolyzed <i>N</i>-benzoyl-L-tyrosine ethyl ester (BTEE).

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 50(1), 145-150, 1984

    日本水産學會

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各種コード

  • NII論文ID(NAID)
    130001543693
  • NII書誌ID(NCID)
    AN00193422
  • 本文言語コード
    JPN
  • 資料種別
    Journal Article
  • ISSN
    0021-5392
  • NDL 記事登録ID
    2974685
  • NDL 刊行物分類
    RA14;RB96
  • NDL 雑誌分類
    ZR26(科学技術--農林水産--水産)
  • NDL 請求記号
    Z18-345
  • データ提供元
    CJP引用  NDL  IR  J-STAGE 
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