Study of lysosomal enzymes in fish muscle tissue - X. Purification of a pepstatin insensitive protease from mackerel white muscle.

• Ueno Ryuji

  Faculty of Bioresources, Mie University

• Sakanaka Kazuki

  Faculty of Bioresources, Mie University

• Ikeda Seiji

  Faculty of Bioresources, Mie University

• Horiguchi Yoshishige

  Faculty of Bioresources, Mie University

Bibliographic Information

Other Title

• マサバ普通肉におけるPepstatin insensitive proteaseの分離・精製
• マサバ普通肉におけるPepstatin insensitive proteaseの分離・精製〔英文〕
• マサバ フツウ ニク ニ オケル Pepstatin insensitive

Abstract

A pepstatin insensitive protease was purified from mackerel white muscle by a combination of acid treatment, ammonium sulfate fraction, Q Sepharose Fast Flow ion exchange chromato-graphy, Cellulofine GC-200 gel filtration, hydrophobic chromatography on Phenyl-Superose column, chromatofocusing on Mono P column, re-chromatofocusing on the column, and ion ex-change chromatography on Mono Q column. The specific activity of the enzyme increased about 7, 750-fold in 1.6% yield, and the final preparation proved to be homogeneous on polyacryl-amide slab gel electrophoresis.

Journal

• NIPPON SUISAN GAKKAISHI

  NIPPON SUISAN GAKKAISHI 54 (4), 691-697, 1988

  The Japanese Society of Fisheries Science

Keywords

• Aquatic Science

Details

• CRID: 1390282681391376256
• NII Article ID: 130001544428
• NII Book ID: AN00193422
• DOI: 10.2331/suisan.54.691
• COI: 1:CAS:528:DyaL1cXksFensbY%3D
• ISSN: 1349998X
• ISSN: 00215392
• NDL BIB ID: 3183995
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3183995
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed