コイ筋小胞体の精製およびその性状

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タイトル別名
  • Purification and characterization of carp sarcoplasmic reticulum.
  • コイ筋小胞体の精製およびその性状〔英文〕
  • コイ キン ショウホウタイ ノ セイセイ オヨビ ソノ セイジョウ エイブン

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Fragmented sarcoplasmic reticulum (FSR) was separated from carp ordinary muscle and purified into light (LSR), intermediate, and heavy sarcoplasmic reticulum (HSR) fractions by a sucrose gradient method. Electron microscopy disclosed that most vesicles in FSR fraction were round-shaped, with a few elliptical and asymmetric vesicles. LSR fraction was rich in elliptical and tubule vesicles, while HSR fraction showed many round vesicles with feet structures.<br>Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate revealed that these fractions had a 97, 000 (97k) Da component as the major. Two ?? 70kDa and one each of 35kDa and 10kDa components exhibited a Ca2+-binding ability in 45Ca autoradiography.<br>Ca2+-ATPase and Ca2+ uptake activities of carp FSR were highly dependent on temperature, both being reduced with decreasing reaction temperature.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 55 (9), 1641-1648, 1989

    公益社団法人 日本水産学会

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