キンギョ耳石の時刻描記法:走査型電子顕微鏡による観察

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タイトル別名
  • Time-marking methods for scanning electron microscopy in goldfish otoliths.
  • キンギョ耳石の時刻描記法:走査型電子顕微鏡による観察〔英文〕
  • キンギョ ジセキ ノ ジコク ビョウキホウ ソウサガタ デンシ ケンビキョウ

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抄録

To produce time marking on otoliths for scanning electron microscopy, young goldfish Carassius auratus were treated with one of the following: tetracycline, ammonium chloride, Diamox, sufocation, starvation, or cold shock. Of those, tetracycline, 1% ammonium chloride and 7 days' starvation produced almost no special markings in the otoliths (lapilli). Didmox (200mg or 500mg/kg) and 30 minutes' suffocation tended to leave markings in the otoliths. However it seems difficult in nature to separate these markings from other check rings formed by unknown events before and during the experiments. The intraperitoneal injection of 3% ammonium chloride also produced a stress marking but this treatment resulited in a high mortality of 50%. Cold shock (23 to 4.2°C) produced a well-defined marking in the otolith and it was easy to discern this marking from other kinds of check rings. The formation rate of the first-order rings in the otoliths was calculated as 0.94 per day by using the experimentally induced markings.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 48 (9), 1225-1232, 1982

    公益社団法人 日本水産学会

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