Simultaneous Detection of Some Yersinia enterocolitica serovars from Food and Human Samples by PCR after IMS-Plating

• UEDA SHIGEKO

  Laboratory of Hygiene, Kagawa Nutrition University

• KUWABARA YOSHIHIRO

  Laboratory of Hygiene, Kagawa Nutrition University

Abstract

After the immunomagnetic beads prepared separately for the capture of Yersinia enterocolitica 03, 05, 27, 08 and 09 were mixed at an equal ratio, the mixemagnetic bead suspension was used for the simultaneous detection of these serovars by IMS-CINplating followed by PCR. The proposed method was shown to be capable of detecting specificallyand simultaneously 03, 05, 27, 08 and 09 from food and fecal samples within 2 days if anyof these bacteria were present at more than 10⁰ cfu/ml in the sample suspension.

Journal

• Biocontrol Science

  Biocontrol Science 10 (3), 111-115, 2005

  The Society for Antibacterial and Antifungal Agents, Japan

Keywords

• <I>Yersinia enterocolitica</I>
• IMS
• PCR
• Food

Details

• CRID: 1390282679440070016
• NII Article ID: 130001642934
• DOI: 10.4265/bio.10.111
• ISSN: 18840205
• ISSN: 13424815
• Web Site: https://search.jamas.or.jp/link/ui/2007001783
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed