Development of A Medical Sensor for Monitoring of Endotoxin Concentration in Dialysate.

• YOTSUMOTO M.

  Waseda Univ.

• HIMI N.

  Waseda Univ.

• YOSHIMI Y.

  Waseda Univ.

• KANAMORI T.

  Waseda Univ.

• SAKAI K.

  Waseda Univ.

Bibliographic Information

Other Title

• 連続測定型エンドトキシンセンサの開発

Abstract

Endotoxin concentration in the diallysate can be continuously monitored with the medical sensor by the Limulus test we have proposed. The objective of the present study is to change TOXICOLOR to ENDOSPECY as a suitable color-producing reagent, and to improve the washing of reaction tube and the mixing of reagent and sample for endotoxin measurements of greater precision. Batchwise reaction experiments were first performed to obtain reaction time, producing a miniunm reaction time of 12min required for appropriate measurements of endotoxin concentration that was the same as that obtained with TOXICOLOR. The time to attain equilibrium after stepwise injection of the sample into the device was 15min at a reaction time of 12min. Measurement precision was greatly enhanced when impurities contaminating the reaction tube were removed by fully washing it with sodium ethoxide, hydrochloric acid and pure water for injection. In conclusion, the improved method is capable of contiunous and exact measurements of endotoxin concentration ranging from 0 to 20pg/ml.

Journal

• Jinko Zoki

  Jinko Zoki 22 (3), 1036-1039, 1993

  JAPANESE SOCIETY FOR ARTIFICIAL ORGANS

Keywords

• medical sensor
• endotoxin
• dialysate
• limulus test
• color-producing reagent

Details

• CRID: 1390001204955695872
• NII Article ID: 130001732514
• DOI: 10.11392/jsao1972.22.1036
• ISSN: 18836097
• ISSN: 03000818
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed