-
- 上原 喜八郎
- Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University
-
- 梅本 準治
- Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University Department of Pharmacy, Faculty of Science and Engineering, Nihon University
-
- 米沢 司郎
- Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University Radiation Center of Osaka Prefecture, Division of Medicine and Hygiene
-
- 大久保 修司
- Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University Kyowa Hakko Company
書誌事項
- タイトル別名
-
- Behavior of Acetyl Groups Combined with Sulfhydryl Groups in Protein
- I. Acetylation of Sulfhydryl Groups in Yeast Alcohol Dehydrogenase
この論文をさがす
抄録
It was found that highly purified crystalline yeast alcohol dehydrogenase (YADH) was still contaminated with a small amount of thiolesterase. Separation of these two enzymes from each other was performed by DEAE-cellulose column chromatography. YADH preparation resulted from this treatment was found to be homogeneous by disc electrophoresis and chromatography on hydroxylapatite column.<br>Treatment of pure YADH with O, S-diacetylthiamine (DAT) in 0.05M phosphate buffer at pH 6.0 and 30° resulted in 50% inactivation of the enzyme with concomitant loss of two SH groups per mole of the protein. Reaction of the DAT-inactivated YADH with hydroxylamine resulted in full reactivation accompanied by restoration of SH groups to the level of the native enzyme.<br>These results supply evidence that the change in enzymatic activity that occurs upon treatment of YADH with DAT is the consequence of acetylation of two SH groups essential for activity per molecule of the enzyme.
収録刊行物
-
- THE JOURNAL OF VITAMINOLOGY
-
THE JOURNAL OF VITAMINOLOGY 17 (3), 128-134, 1971
日本ビタミン学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001206322799616
-
- NII論文ID
- 130001749689
-
- NII書誌ID
- AA00247734
-
- COI
- 1:CAS:528:DyaE38XjtFOqsw%3D%3D
-
- NDL書誌ID
- 8506353
-
- ISSN
- 00225398
-
- PubMed
- 5140837
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可