EFFECT OF ISOFLAVONE ON HUMAN ENDOMETRIAL CANCER CELL LINES IN VITRO

• MIYAMOTO Shingo

  Department of Obstetrics and Gynecology, Showa University School of Medicine

• YANAIHARA Atsushi

  Department of Obstetrics and Gynecology, Showa University School of Medicine

• MITSUKAWA Kaori

  Department of Obstetrics and Gynecology, Showa University School of Medicine

• NAGATSUKA Masaki

  Department of Obstetrics and Gynecology, Showa University School of Medicine

• OKAI Takashi

  Department of Obstetrics and Gynecology, Showa University School of Medicine

Bibliographic Information

Other Title

• イソフラボンのヒト子宮内膜癌細胞株増殖能に対する効果
• イソフラボン ノ ヒト シキュウナイマク ガン サイボウシュ ゾウショクノウ ニ タイスル コウカ

Abstract

The objective of this study was to examine the action of isoflavone on human endometrial cells. We determined the effect of a daidzein-rich isoflavone aglycone mixture (Dr. AglyMax^®) on the growth of hormone-dependent endometrial cancer cells (ISHIKAWA cell) in vitro. ISHIKAWA cells were plated into 96-well micoplates, treated with Dr. AglyMax^TM, and incubated for 24h. Cell proliferation was analyzed by ELISA using specific antibodies. We compared cell proliferation between Dr. AglyMax^®at a concentration of 100 μM and a control without Dr. AglyMax^®. Total RNA was isolated from cultured cells by the acid guanidine thiocyanatephenol/chloroform method. We also examined the changes in approximately 47, 000 gene expressions using a microarray method. Cell proliferation of the Dr. AglyMax^®-treated group was lower than that of the control group (67%) (p<0.01) . A total of 1165 genes were down-regulated and 1114 genes were up-regurated following treatment with 100 iM Dr. AglyMax^® . Our study suggests that daidzein-rich isoflavone aglycones inhibit endometrial cell growth and these genes, which are involved in steroid metabolism and cell cycles, are likely related to inhibition of cancer cell growth.

Journal

• Journal of The Showa Medical Association

  Journal of The Showa Medical Association 67 (1), 79-86, 2007

  The Showa University Society

Keywords

• isoflavone
• endometrial cancer
• cell proliferation
• gene expression

Details

• CRID: 1390001204834574080
• NII Article ID: 130001820221
• NII Book ID: AN00117027
• DOI: 10.14930/jsma1939.67.79
• COI: 1:CAS:528:DC%2BD2sXntlKhsrw%3D
• ISSN: 21850976
• ISSN: 00374342
• NDL BIB ID: 8861520
• Web Site: http://id.ndl.go.jp/bib/8861520
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8861520
• Web Site: https://search.jamas.or.jp/link/ui/2007289532
• Data Source:
  • JaLC
  • NDL
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed