Quantification of Cyanamide in Young Seedlings of <i>Vicia</i> Species, <i>Lens culinaris</i>, and <i>Robinia pseudo-acacia</i> by Gas Chromatography-Mass Spectrometry

  • KAMO Tsunashi
    Biodiversity Division, National Institute for Agro-Environmental Sciences (NIAES) Biodiversity Division, National Institute for Agro-Environmental Sciences (NIAES)
  • TAKEMURA Tomoko
    Biodiversity Division, National Institute for Agro-Environmental Sciences (NIAES) Biodiversity Division, National Institute for Agro-Environmental Sciences (NIAES)
  • WASANO Naoya
    Biodiversity Division, National Institute for Agro-Environmental Sciences (NIAES) Biodiversity Division, National Institute for Agro-Environmental Sciences (NIAES)
  • FUJII Yoshiharu
    Biodiversity Division, National Institute for Agro-Environmental Sciences (NIAES) Biodiversity Division, National Institute for Agro-Environmental Sciences (NIAES)
  • HIRADATE Syuntaro
    Biodiversity Division, National Institute for Agro-Environmental Sciences (NIAES) Biodiversity Division, National Institute for Agro-Environmental Sciences (NIAES)

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  • Quantification of Cyanamide in Young Seedlings of Vicia Species, Lens culinaris, and Robinia pseudo-acacia by Gas Chromatography-Mass Spectrometry

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Abstract

We quantified the cyanamide content of young leaves of nine Vicia species, Lens culinaris, and Robinia pseudo-acacia using a modified analytical procedure that made it possible to measure the cyanamide content of a single leaf. Recent molecular phylogenetic analysis suggests that cyanamide is present in V. benghalensis, which is placed in a monophyletic group with cyanamide-biosynthesizing plants, V. villosa and V. cracca; this suggestion was verified.

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