Elevation of Intracellular Ca²⁺ Level by Triclosan in Rat Thymic Lymphocytes : Increase in Membrane Ca²⁺ Permeability and Induction of Intracellular Ca²⁺ Release Elevation of Intracellular Ca2+ Level by Triclosan in Rat Thymic Lymphocytes: Increase in Membrane Ca2+ Permeability and Induction of Intracellular Ca2+ Release

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著者

    • Tamura Ikumi Tamura Ikumi
    • Division of Environmental Symbiosis Studies, Graduate School of Integrated Arts and Sciences, The University of Tokushima
    • Saito Minoru Saito Minoru
    • Division of Environmental Symbiosis Studies, Graduate School of Integrated Arts and Sciences, The University of Tokushima
    • Satoh Masaya
    • Division of Environmental Symbiosis Studies, Graduate School of Integrated Arts and Sciences, The University of Tokushima
    • Yamamoto Hiroshi
    • Division of Environmental Symbiosis Studies, Graduate School of Integrated Arts and Sciences, The University of Tokushima
    • Oyama Yasuo
    • Division of Environmental Symbiosis Studies, Graduate School of Integrated Arts and Sciences, The University of Tokushima

抄録

Triclosan is an antibacterial agent used in household items and personal care products. Because wild animals and humans can harbor this compound in their systems, the toxic effects of triclosan are a possibility and are suspected. Therefore, we examined the effects of triclosan on intracellular Ca<sup>2+</sup> concentration in rat thymocytes by cytometric techniques using fluorescent probes. Triclosan doses of 1-10 μM significantly increased the intensity of Ca<sup>2+</sup>-detecting Fluo-3 fluorescence, indicating an increase in intra-cellular Ca<sup>2+</sup> concentrations. The augmentation of Fluo-3 fluorescence became more profound in a dose-dependent manner after the addition of an external source of Ca<sup>2+</sup>. Conversely, the removal of external Ca<sup>2+</sup> greatly attenuated the triclosan-induced augmentation of Fluo-3 fluorescence. These results suggest that triclosan treatment allows external Ca<sup>2+</sup> to pass through cell membranes. This phenomenon was not specific for Ca<sup>2+</sup> because external Mn<sup>2+</sup> quenched the triclosan-induced augmentation of Fluo-3 fluo-rescence, indicating that triclosan can also mediate Mn<sup>2+</sup> permeation across membranes. Therefore, these results suggest that triclosan increases membrane permeability to divalent metal cations. Furthermore, triclosan induces Ca<sup>2+</sup> release from intracellular stores because the Fluo-3 fluorescence intensitystill increased slightly after triclosan treatment, even under conditions free from external Ca<sup>2+</sup>. Additionally, triclosan did not increase the intensity of Fluo-3 fluorescence when Ca<sup>2+</sup> was depleted from intracellular Ca<sup>2+</sup> stores by A23187 under the external Ca<sup>2+</sup>-free condition. Taken together, these data suggest that micromolar concentrations of triclosan affect intracellular Ca<sup>2+</sup> homeostasis in thymocytes, possibly resulting in cellular malfunction.

収録刊行物

  • JOURNAL OF HEALTH SCIENCE

    JOURNAL OF HEALTH SCIENCE 57(6), 540-546, 2011

    公益社団法人 日本薬学会

各種コード

  • NII論文ID(NAID)
    130001869871
  • NII書誌ID(NCID)
    AA11316464
  • 本文言語コード
    ENG
  • ISSN
    1344-9702
  • NDL 記事登録ID
    023321468
  • NDL 請求記号
    Z54-J464
  • データ提供元
    NDL  J-STAGE 
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